To improve the survival rate of afforestation in the spring, we should grasp the following points: 1. In the early spring of planting time, the soil moisture content was relatively good, and the ground temperature gradually increased. After planting the roots of Miao Miao, it germinated and the survival rate of planting was high. Therefore, it is better to plant trees in the spring rather than later. Digging pits can be planted from the beginning of March. 2. Selection of tree species to choose seedlings must be done: First, choose to grow robust, full lignification, no pests and diseases, uniform seedlings specifications; second is to raise the seedling soil ball to be large, seedling roots to be intact, with fibrous roots, the main root fracture is neat The third is to select large seedlings with a diameter of breast diameter of more than 3 cm and a seedling height of 3.5 meters or more for two years or more. 3. Planting techniques 1 seedling treatment. Before planting, one must cut the lateral branches of the seedlings, cut off all the lateral branches on the seedlings, leave 2-3 buds, and remove the competition branches; the second is to soak the seedlings in the water, avoid drying and winding the seedlings, especially for long-distance transportation. Seedlings must be soaked in water for 24 hours. 2 digging pit specifications. It is required to excavate planting pits with a length, width and height of 80 cm square. The depth of planting should be 2-3cm beyond the original soil mark at the root of the seedlings, and it should be properly planted under drought conditions. The soil may be slightly moist and sticky. 3 planting methods. At the time of planting, the planting techniques of “three burying and two cultivating†were used to highlight the “three major onesâ€, namely, pits, big seedlings, big water, and compaction. When the filling depth is about 2/3, the seedlings are lifted slightly, filled with soil until the pit is full and then stepped on. Finally, the water is poured into the roots and a layer of loose soil is placed on the pit surface to prevent the evaporation of soil moisture. 4. Management work 1 timely watering. According to the weather and soil moisture, timely watering the newly planted saplings. Usually, the newly planted saplings will ensure that water is poured 6 times before harvesting in the first year. And at any time to check whether there is lodging, tilting or rooting phenomenon, if found should immediately righting, filling and tamping. 2 pay attention to harm. After planting, in order to prevent trees from being damaged by pests or livestock, the trunk should be painted white or coated with a bactericide.
The enzyme-linked immunosorbent assay (ELISA) (/ɪˈlaɪzÉ™/, /ËŒiËˈlaɪzÉ™/) is a commonly used analytical biochemistry assay, first described by Engvall and Perlmann in 1971.[1] The assay uses a solid-phase enzyme immunoassay (EIA) to detect the presence of a ligand (commonly a protein) in a liquid sample using antibodies directed against the protein to be measured. ELISA has been used as a diagnostic tool in medicine, plant pathology, and biotechnology, as well as a quality control check in various industries.
In the most simple form of an ELISA, antigens from the sample are attached to a surface. Then, a matching antibody is applied over the surface so it can bind to the antigen. This antibody is linked to an enzyme, and in the final step, a substance containing the enzyme's substrate is added. The subsequent reaction produces a detectable signal, most commonly a color change.
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