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Identify the learning principle of Morris water maze experiment
Morris Water Maze Experiment
principle: Â A mouse or rat can learn to swim in a water tank and find an experimental method for hiding in an underwater escape platform. Since there are no accessible clues to mark the position of the platform, the effective positioning ability of the animal requires the use of a structure outside the tank as a clue. The labyrinth consists of a circular pool, an automatic camera and an analysis system. The automatic image acquisition and processing system is mainly composed of a camera, a computer, and an image monitor. The animal starts the monitoring device after entering the water, records the animal's motion track, and automatically analyzes the report after the test is completed. parameter.
Detection Indicator: Â The experimental procedures include: (1) Place navigation, which is used to measure the ability of mice to acquire water and maze learning and memory. The experiment lasted for 4 days, and each training was performed once in the morning and afternoon, for a total of 8 times. The experiment observed and recorded the route map and time required for the mouse to find and climb the platform, that is, record its latency and swimming speed. (2) Spatial probe test, which is used to measure the ability to maintain the memory of the platform space after learning to find the platform. After the positioning navigation experiment is completed, the platform is removed, and the water is placed in the water from the same water inlet point, and the time of the first arrival at the original platform position and the number of crossing the original platform are measured.
Advantages and Disadvantages: Morris Water Maze is a more objective evaluation method for learning and memory function in the world. The Morris water maze was used to detect spatial memory learning ability. The main advantage of the water maze compared with the radial arm maze is that 1 in the water maze, the time required for animal training is shorter (1 week), while the arm maze requires several weeks of training time; 2 clues in the maze For example, the odor can be eliminated; the three large dose-effect studies can be performed within one week; 4 the computer can be used to establish an automatic image acquisition and analysis system, which can be used to generate corresponding histograms and runs based on the collected data. The trajectory map is convenient for researchers to further analyze and discuss the experimental results to study the movement or motivation problems of rats; 5 animals can not be fasted in the experiment. In theory, the water maze experiment is an aversive-driven experiment and the arm-maze experiment is an appetite-driven experiment.
Conclusion
At present, the research on learning and memory is progressing very rapidly, and various theories of learning and memory are constantly emerging. Animal models designed in accordance with these theories are also constantly emerging. Various advanced experimental techniques such as LTP and ERP of neuroelectrophysiological techniques and fMRI of brain imaging techniques have been applied to the study of learning and memory, which has opened up a new way for the study of learning and memory. Therefore, how to combine traditional behavioral research methods with the latest research techniques to provide a broader idea for the study of learning and memory is the development direction of behavioral research in learning and memory in the future.