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Seven characteristics establish the dominant application status of 300Ã… C18
In recent years, with the implementation of genomic and proteomic programs, the rapid analysis, qualitative and quantitative requirements for different proteins are more demanding. Reversed-phase liquid chromatography is fast, simple, sensitive, reproducible and high resolution. People's attention has become a necessary and indispensable analytical tool and used in the separation of peptide drugs. The combination of reversed-phase liquid chromatography and various mass spectrometry techniques has also become an important means and development direction of protein structure analysis.
The InnovationTM 300Ã… C18 developed by Chrom-Matrix relies on seven innovative features to determine its position in the application.
First, the stability of the InnovationTM 300Ã… C18 column in the 100% aqueous mobile phase often involves very polar peptides in bioseparation applications. If only polar peptides are available, they can be isolated using InnovationTM HP Amide Hydrophilic Chromatography, but in practice it is often a mixture of polar peptides and different hydrophobic peptides. In particular, peptide mapping under full-blind conditions, it is hoped that the linear gradient can start from the 100% water mobile phase, so as to obtain the information of different polar peptides after enzyme digestion. Chrom-Matrix's InnovationTM 300Ã… C18 column can be 100% The stability in the water mobile phase is very good.
Second, the basic peptide has a symmetrical peak shape on the InnovationTM 300Ã… C18 column. The InnovationTM 300Ã… C18 eliminates the acidic effect of the silanol group through the mature supercritical fluid capping technology, thus becoming a "family" High quality liquid chromatography column with pure reverse phase mechanism. Thus all polypeptides, including difficult polypeptides, are able to obtain symmetrical peak shapes. In contrast, many alkaline peptides are severely tailed or even missing on the main competitor's column.
Third, LC-MS analysis of peptides on the InnovationTM 300Ã… C18 (C8 or C5) column eliminates the need for trifluoroacetic acid. Trifluoroacetic acid is often used as an ion in the separation or purification of peptides or proteins by reversed-phase high performance liquid chromatography. The peak shape is maintained for the reagent. If only UV detection is used, although there is no problem, as LC/MS technology becomes more and more popular, many problems in life phenomena are increasingly dependent on the analysis of the structure and composition of proteins by trace analysis of LC/MS. (Proteomics), scientists urgently need to avoid the use of trifluoroacetic acid in peptide or protein LC-MS analysis, because trifluoroacetic acid severely reduces the sensitivity of mass spectrometry detection. InnovationTM 300Ã… C18 (C8 or C5) maximizes the elimination of the acidic effects of silanol radicals through proven supercritical fluid capping technology, thus becoming a "virtual" high-quality, purely reversed-phase HPLC column , LC-MS analysis of peptides on the InnovationTM 300Ã… C18 (C8 or C5) column eliminates the need for trifluoroacetic acid.
Fourth, the InnovationTM 300Ã… C18 column does not leak in LC-MS analysis, and many of the problems in life phenomena are increasingly dependent on LC/MS for trace analysis of changes in protein structure and composition (proteomics). If the column leaks in the LC-MS analysis, it will seriously interfere with the analysis. The InnovationTM 300Ã… C18 column has no leakage in LC-MS analysis, which is very important in LC-MS analysis, especially proteomics!
Fifth, peptide mapping resolution, peptide mapping is one of the most classic and most effective analytical methods for protein chemistry. The InnovationTM 300Ã… C18 column uses a single-function, single-layer, high-density bond that is then capped with a supercritical fluid, so all peptides are well-retained and symmetrical. As a result, the peptide map has the highest resolution, thereby maximizing information on protein sequence, structure and purity.
Sixth, the column has a long life, and the separation and purification of the polypeptide often uses trifluoroacetic acid. After solid phase polypeptide synthesis, the polypeptide is often cleaved using HF or trifluoroacetic acid. Residual acid often damages column life. The InnovationTM 300Ã… C18 column uses a single-function, single-layer, high-density bond that is then capped with a supercritical fluid to maximize column stability and longer column life.
Seventh, the reproducibility between batches of InnovationTM 300Ã… C18 columns, biopharmaceuticals represented by proteins is a much more complex process than small molecule pharmaceuticals. The quality control of each step of the process and the final product cannot leave the protein (polypeptide) high performance reverse phase chromatography product. Reproducibility between biopharmaceutical batches is poor. Repeatability between batches of high performance reversed-phase chromatography products must be ensured, otherwise additional uncertainties can be added, further complicating the already complex quality control. The difference between the production batches of InnovationTM 300Ã… C18 columns is less than 1%.
Chrom-Matrix's innovative R & D Innovation TM 300Ã… C18, is based on years of research and development team for in-depth study of customer applications, it's better to play a reverse phase liquid chromatography effective separation of mixtures of various polypeptides, especially for molecular weight of not The separation, purification and identification of large proteins and peptides have been widely used in protein and peptide research, effectively meeting the needs of separation and purification in life science research.