Bone samples were cleaned of adherent soft tissue and osteoblasts isolated by two methods. a. The bone was cut into small pieces (2 mm × 2 mm), washed in Ca 2+ - and Mg 2+ -free digestion's solution (10 min), and then sequentially digested (1 mg/ml trypsin, 10 min; 2 mg/ml Dispase, 20 min; and 3 mg/ml collagenase, 2 × 30 min) in digestion's solution at 37°C. Cells released by the collagenase digestions were washed and grown to confluence in 25 cm culture flasks (Falcon) in Primary cell culture system supplemented with 10% fetal calf serum (FCS). Incubations were carried out at 37 ° C in a humidified atmosphere of 5% CO 2 / 95% air; the medium was changed every 2-3 days. b. Bone samples were cut into small pieces and plated into 5 cm Petri dishes containing 4 ml primary cell culture system supplemented with 10% FCS. Bone cells were allowed to grow out from the explants until confluent; the medium was changed every 2-3 Days. Led Light Head,Siren Amplifier,Ambulance Speaker,Electronic Siren Amplifier jiangyin chenyi medical technology co.,ltd , https://www.chenyimed.com
Isolation of osteoblasts from human bone.
Isolation of osteoblasts from human bone.