The perfection of traditional Chinese medicine research and SCI

This article has been brewing for a long time. I have never dared to write. Worried that your accumulation is not enough. During this time, I saw that the Chinese medicine-made cheeses, whether they were brought to the country or not, were not obtained. In the design of the subject, there will always be some embarrassment. So, today, I am brave enough to write this article. I hope that I can give you a hint for the Chinese medicine research.

In the "where is the ultimate way out of the disease", Ji Bo said that Western scientific research uses the theory of reduction. Looked closer. So everyone sees the SCI article, the more detailed it is, the higher the score. Eastern science is holistic. Going bigger. Traditional Chinese medicine is really good to study. It is necessary to learn the "Yellow Emperor's Internal Classic" first. This is the medical theory of Chinese medicine. But this is hard. Therefore, it is very difficult to learn Chinese medicine. There are even fewer people who can understand. In addition, as mentioned in Buddhism, "the era of the end of the law, the sorcerer's statement such as Henghesha" caused misunderstanding of Chinese medicine.

In the study of traditional Chinese medicine, there is a saying that "the Chinese medicine is divided into a branch of the Chinese medicine, and it is the group's operation. It is certainly not possible to study with Westernization theory." In the past, Ji Bo also felt reasonable. However, think about it now and look at Western research. Look up the literature, you will find that the same disease, the cause of the disease is not a gene. However, in a high-scoring article, only one major gene will be made. Do you understand? Traditional Chinese medicine is a group operation, but each component has its own role. This function can be large or small in the overall treatment, but its function exists. We use Western methods of covenant research to link this component to its function. In this way, through a lot of research, you can understand the whole party more comprehensively. Thereby providing a "scientific basis" for the modernization of traditional Chinese medicine. Sorry, the "scientific basis" here is quoted by Ji Bo, which is a Western scientific system. After each component and its function are clearly clarified, if you integrate it, you need to use holism. The theory of decimability and holism is actually the yin and yang of one thing, and cannot be separated. Special attention should be paid to the Chinese medicine research. Don't negate the theory of covenant, and don't abandon the omnipotence.

Having said that, I want to explain it to you from a theoretical point of view. This article is a theoretical system and method from the theory of covenant to tell everyone how to study Chinese medicine. Huang Di Nei Jing, Ji Bo did not understand, did not dare to speak.

The ultimate goal of clinical disease research is to discover the causes of disease development. This reason is the candidate drug target.

The study of western medicine is to find a drug based on the candidate drug target after having a candidate drug target.

The study of traditional Chinese medicine is the first medicine. Then, look at the mechanism, for this effect will occur, who is the drug target. Have a medicine first, then find a drug target.

Therefore, the study of traditional Chinese medicine, in fact, the most important thing is to find a functional target. The drug target here is the functional gene in the disease. Therefore, at the beginning of the design of traditional Chinese medicine, it is recommended to first look at the "question design ideas", how the drug target is proved. This is the premise of medicine.

Chinese medicine research, Ji Bo is divided into three parts. 1, drugs. 2, effectiveness. 3. Mechanism (drug target).


First, the drug
In this regard, it is to explore what effective drugs are. The traditional Chinese medicines currently studied can be divided into compound, subtractive side, square, and monomer.

Everyone noticed that the monomer is actually the same as Western medicine. Many plant-derived chemotherapeutic drugs are actually in this category.

From the research point of view, the monomer is better than the unilateral, the unilateral is better than the decomposed party, and the decomposed party is better than the compound.

Therefore, in the aspect of drugs, we are looking for a teacher to do drug separation. Seek cooperation.

In this regard, the separation of monomers from botanicals and the identification of structures is the SCI article. If you add the latter two steps to study the monomer and drug target, it is not enough.

Six enzymes from mayapple that complete the biosynthetic pathway to the etoposide aglycone. Science. 2015.

This article was published on science this year. Etoposide is a clinical chemotherapeutic drug. The precursor of this drug is from mayapple. When the lecture was given, a director told Ji Bo that this mayapple is the traditional Chinese medicine octagonal lotus.

What this article does is how this precursor is synthesized in plants. In fact, it is possible to send science, in addition to studying important drugs, and more importantly, preparing for the mass production of this drug precursor in the future.

Second, effectiveness
In the Jibo lecture, one reason for believing in traditional Chinese medicine is that Chinese medicine has been a summary of human experiments for thousands of years. If you calculate from Yandi Shennong, it is almost 5,000 years.

However, everyone notices that whether Chinese medicine is effective or not, from the perspective of research, clinically effective, and the mechanism is unclear, it is difficult to pass FDA certification. This is the problem facing Chinese medicine now.

The most important here is the data of animal models and cell models.

1 , animal model
Disease modeling. Single disease modeling is a good topic. On this basis, drugs are added to assess the effectiveness of the drug.

2 , cell model
The cell model is simple in background, easy to operate, and easy to evaluate. It should be noted here that the detection index on the cell needs to be consistent with the detection index when the disease function gene is studied. The general recommendation is an indicator of proliferative apoptosis.
It is recommended to promote the direction of apoptosis, because it is good to do. It is noted that in the lecture on "Clinical Disease Research Thinking", the first step in scientific research is to determine the cell model and detection indicators of the study. In this process, there are two principles.

a , what are the cell models and test indicators. A high score reference is required. That is, the relationship between the cell model and the test indicators I use and the disease is recognized by everyone.

b , can I do it ? This is very important. Many people have a good design, but either the cells can't be raised or the specific experiments can't be done. In the current natural application, many people in the first-instance expert evaluate the feasibility. Because everyone sees more literature, what to do, look at the high-scoring literature. But whether the actual situation can really be done needs to be combined with the background of the researchers to assess the feasibility.

Animal models and cell models are tested and the drug is functionally phenotypical. Then go down. Of course, if you are in a hurry to send SCI, as long as there is innovation here, you can send SCI.

Third, the mechanism (drug target)

This step is to show that the drug is a functional phenotype. In fact, it is to find the disease function genes regulated by drugs.

In disease research, we hope to find the disease function genes that are low in the normal group and high in the disease group. In other words, the high expression of this gene leads to the development of the disease. Such genes have a higher success rate as drug targets.

In the study of traditional Chinese medicine, we hope that the performance of the drug target is low in the normal group, high in the disease group, and low in the disease treatment group. Ji Bo called this kind of gene a "mountain" glyph. The difference in expression between the three groups presented the "mountain" shape.

In the normal group, the disease group, and the disease treatment group, the "mountain" gene. Jibo is divided into three categories.

1 , class 1 . It is a known disease-associated signaling pathway gene.
This kind of gene is actually the most common type of SCI in Chinese medicine. The score is not high.

2 , 2 categories. It is a disease-related important disease gene.
If you do this kind of gene, the score will be higher. "P53 is a tumor suppressor gene-transcriptional regulation-methylation acetylation-drug target". The nature article in this article is classified into two categories. In the Jibo log, all the articles with the title "drug target" are expected to be seen. If you are doing the screening process yourself, there are these genes in the "Mountain" glyph. Then do these genes. Because the articles shared by Jibo are nature. And basically this year. It is very new to use these genes as drug targets for their own research drugs.

3 , 3 categories. Unknown disease-related genes.
In fact, when Jibo helped people design projects, if they were anxious to take the country's natural SCI, then they would do 1 class 2 drug targets. But in fact, the three types of drug targets are the best. Because innovation is the highest. The drug target is newly discovered, and the relationship between the drug and the drug target is also newly discovered.

OK. Regardless of whether it is a Class 1 or Class 3 drug target, drug target identification is required in the study. The identification of drug targets is actually the three major blocks of our disease function gene research, plus the "relevance of drug targets and drugs."

Three major blocks of disease function gene research, clinical relevance, function, and mechanism. If you want to know this part, look at the "question design ideas."

The relevance of drug targets to drugs. In fact, when we do the disease function gene mechanism, it is actually the same idea to clarify the relationship between the A gene and the B mechanism. Replace the A gene with our drug.

1 , molecular correlation
After the drug is added, the drug target changes.

2 , functional relevance
a . After dosing, the drug target drops and the cells have a functional phenotype. Then interfere with the drug target alone to see if the functional phenotype is consistent with dosing. In fact, if the drug targets here are Class 1 and Class 2, this experiment has already been done by others. We need to repeat it.

b . After the drug is added, the drug target is lowered and the cells have a functional phenotype. Then, under the premise of dosing, the drug target is overexpressed to see if the cell phenotype is restored. This step is actually a lot of nature will do. To prove that the function of the drug is indeed through this target. This is the rescue experiment.

3 , direct interaction
I am sorry to say to everyone here. We are talking about the current drug target, which is the effective drug target of the drug. Whether it is a direct binding target, not necessarily. If the drug and the drug target are to be directly interacted with each other, the drug needs to be a monomer. Look for binding proteins from the monomer. And the protein is a disease function gene. If you can make it, just cast nature medicine.

Avenue to Jane. In the study of disease function genes, one sentence is "the A gene affects the D function of C disease by regulating the B signaling pathway." The innovative A gene, the relationship between the A gene and the disease, was first reported.

For Chinese medicine research. To sum up one sentence is "drug E inhibits the development of C disease by regulating the A gene to influence the B signaling pathway." Where is the innovation?

1. The relationship between drug E and disease is known.
This situation is suitable for the starters of scientific research. Because the relationship between drugs and disease is known, that is, animal models or cell models in assessing effectiveness are known. Then we only need to find a drug target.

a , the relationship between the A gene and the disease is known.
In this case, drug E and disease are known. The relationship between the A gene and the disease is known. The innovation is that the relationship between drug E and gene A is the first report. SCI can be issued, but the innovation is small and the score is not high. Unless the drug is a monomer, the monomer binds directly to the A gene.

b . The relationship between gene A and disease is unknown.
This is the direction suggested by Jibo. Because the relationship between the single A gene and the disease proves to be good, it is a good subject. If you follow the "question design ideas", do a good job of 10 points or more. This belongs to the study of disease function genes. On this basis, the work of the drug is added. Innovation is guaranteed. In fact, many Chinese medicines that have won the bid are naturally the place to be.

2. The relationship between drug E and disease is unknown.
In this case, it is necessary to first focus on the disease model and effectiveness assessment.

Because the relationship between drug E and disease is new. If the A gene is old, the story is easy to understand. If it is new, it is innovative, but it needs to be well designed to prove it.

Here, Ji Bo tells you something. There is a teacher who, according to the subject of the winning bid, is designed to be able to publish SCI articles. Ji Bo proposed to find new drugs.

There is a saying in Chinese medicine that "helping the righteousness and evil spirits." Supporting the right to raise. Exorcism is poison. This teacher is a tumor. Models are easy to get. On the drug, Ji Bo suggested to find drugs to exorcise evils in Chinese medicine. Such drugs are easy to manipulate on cell and animal models for research on tumors. In the past two days, on the WeChat, Mr. Zhang Tingdong got the "Qiushi" award. Arsenic trioxide is a type of exorcism and poison in Chinese medicine. There is also Tripterygium. Let's find it.

If you want to find someone to discuss a topic, you can find Jibo. Because we want to make your research more professional and simpler. At the same time, Ji Bo also hopes that everyone will pass on traditional Chinese medicine.

PS: I hope that friends who have more exchanges with Jibo in learning and experimentation can pay attention to Jikai Gene WeChat and reply to the words “Jibo”.

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