Techniques of Artificial Seedling of Triploid Monoploid in Dalian Bay

I. Selection and promotion of pro-shellfish Selection of Dalian Bay oysters aged 2 to 3, remove the attachments on the shells, and then hang them in the cultivation pool. The parent-feeder can use floating cages or cage-type cages. The stocking density was 50/m3 to 60/m3. The pool water was heated to 1°C every day until the water temperature reached 20°C to 22°C. After constant temperature incubation, the effective accumulated temperature reached 450°C or more and the progenitors were fully mature and mature.
2. Egg-picking and fertilization The pro-scallops were disinfected in 10ppm potassium permanganate solution for 5 minutes, then rinsed with clean seawater, prodded from their ligaments with a scalpel, and cut off the adductor muscles to cut the gonads immediately. The pipette sucks a drop of the scratched gonad and drops it on a slide containing a drop of water. If the gonadal cells spread out immediately, it is an egg; if the gonads are not easy to spread out and become smokey, it is a sperm. The ovary was removed and crushed. Superfluous impurities and tissues were filtered with a 250 mesh sieve. Artificial spermatozoa were added for artificial insemination. The agitation was performed for about 30 minutes. Under a microscope, approximately 40% to 50% of the first polar body of the fertilized egg appeared. After that, dimethylaminopurine (6-MAP) was added and transferred to the hatching tank after 15 minutes to 20 minutes.
Third, the larvae cultivate fertilized eggs hatching density of 10 / mL ~ 15 / mL, and in the pool water by adding 0.5ppm ~ 1.0ppm EDTA solution, 3.0ppm of antibiotics, about 24 hours can be developed to D-shaped larvae, began to choose Education requires the shells to wrap all the soft parts. When breeding, prevent larvae from mechanical damage, do not allow larvae in cages to be overly dense, and operate lightly. The food organisms are dominated by golden algae, and Platycladis and Chlorella are added at the end of the shell. During the cultivation period, micro-inflating should be performed and water should be changed regularly. The larvae appear eye spots from 18 days to 20 days.
4. When the proportion of eyeball larvae is 60% or more, the attachment base is put in. The attachment base is made of polyethylene plastic cloth and cut to a size of 60cm20cm. The attachment base is placed at a density of 6 blocks per cubic meter to 8 blocks per cubic meter. And the bottom of the pool should also be covered with plastic sheeting. The amount of water exchange should be increased after the attachment base is put in, and 3.0ppm of antibiotics should be applied.
Fifth, after the management of larvae is attached, increase the amount of water exchanged and the amount of feeding, and observe the larvae's growth regularly. After larvae have been cultivated indoors for a period of time, they should be moved to a shrimp pond for intermediate breeding.
During the entire nursery process, secondary sand seawater is used, and the biological requirements of the feed are strict. The application of antibiotics every other day during the larval rearing stage can prevent disease.
Author: Rongcheng Fisheries Technology Promotion Station, Shandong Province

Natural pigment supplier Organic Herb Inc's range of products include: Marigold Extract (lutein, yellow pigment), Sorghum biocolor extract (Sorghum pigment, red), beetroot extract (Beet Red), Ginger extract (gingerol yellow), Black Carrot Extract (red), Purple Kale pigment(red cabbage pigment), Purple Sweet Potato Color, chili extract (capsicum red pigment), Gardenia Extract (Gardenia Yellow) etc, these Natural Colors can be widely used as food additives , beverage additives and dietary supplement etc.

Natural Pigments have grabbed a lot of attention as an alternative of synthetic pigments.The plant extracted color finds its application in a variety of fields, including pharmaceuticals drug production ,nutraceuticals,food production ,dairy, beverages, animal feed ,cosmic and the confectionery segment.Plant extract of natural colors is not only good for coloring but also good for improve wellness as most of them are antioxidants.Organic Herb Inc is a China-based plant extract manufacturing company which takes pride in providing a wide range of high quality environmentally friendly and water miscible pigments from organic plants at competitive market prices.

Natural Colors / Pigments

Natural Colors,Natural Pigments,Carrot Pigment,Gardenia Extract Powder

Organic Herb Inc. , http://www.extracts.pl