Liquid preservation at room temperature: It means that the diluted semen is stored in the environment around 20°C, and the storage time is very short, only 1 to 2 hours. Cryopreservation: The semen is stored in a 0°C to 5°C environment for several days. Special care should be taken to reduce the diluted semen to 5°C slowly. The method is to use a dry towel or double gauze to hold the container containing the diluted semen in 4 to 5 layers and store it in a low-temperature environment (such as a refrigerator).
Collagen is a triple helical protein which can be considered as the bio-glue inside our body; in fact, animal glue can be obtained by boiling the animal skin. Collagen, a major component of connective tissues, exits in the extracellular space of these tissues which are the key reinforcing and bonding materials for all tissues and organs throughout our body, forming rigid structures as such bone, semi-rigid tissues such as cartilage, or soft tissues such as muscle, tendon, skin, ligaments, and cell membranes, etc. There are different forms (fibrillar and non- fibrillar) and types of collagens in the body; Type 1 being the major type constitutes over 90% in our body and is the major component in skin, tendon, vascular ligature, organs, bone (main component of the organic part of bone). Because collagen is an essential building material of all tissues and organs, it has many medical uses, such as in cardiac (hear) applications, cosmetic surgery, bone grafts, tissue regeneration, reconstructive surgical uses, and wound healing care.
Collagen is created inside fibroblast cells, and this process is needed to support the creation and repair of the body`s connective tissues. However, the biological process starts to breakdown when we are aging, normally after we reach the age of late 20s or early 30s. Because collagen from natural sources such as animal, fish scales or plant contain essentially the same amino acid compositions (glycine, proline, alanine, hydroxyproline, glutamic acid, arginine, aspartic acid, serine, lysine, leucine, valine, threonine, phenylalanine, isoleucine, etc.) as human collagen, supplement the body with the natural collagen, either by dermal application or through oral ingestion, can help rejuvenate collagen creation process to support the repairing of aging connective tissues in our body, particularly those in our skin, and to reverse or slow down the aging process for a more youthful appearance.
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Cryopreservation means that the semen is diluted with an antifreeze (such as dimethyl sulfoxide and glycerol) and stored in a cryogenic environment at -79°C (solid carbon dioxide) or -196°C (liquid nitrogen). Sperm can be stored for a long time at this temperature. 
