High-speed refrigerated centrifuges are essential separation devices in the fields of medicine, life sciences, and agricultural sciences. I. Working principle When the suspension containing fine particles is left still, the suspended particles gradually sink due to the action of the gravity field. The heavier the particles, the faster the sinking, whereas the particles with a lower density than the liquid will float. The rate at which particles move under the gravitational field is related to the size, morphology, and density of the particles, and is related to the strength of the gravitational field and the viscosity of the liquid. use: It is suitable for the separation, concentration and preparation of viruses, cells and biological macromolecules. It is an indispensable tool for cell biology and molecular biology research. Domestic brands: Xiangyi, Jingli, Yingtai............ Foreign brands: American Beckman, Germany SIGMA, Japan Hitachi......... High-speed refrigerated centrifuge operating procedures and precautions First, the operating procedures: (1) Place the sample in the same amount in the test tube and place it symmetrically into the rotor; (2) Tighten the shaft nut, cover the door, connect the instrument to the power supply, the measured speed indicator light is on, and the digital tube displays “00000â€, indicating that the instrument is powered on; (3) Press the operation key to start the instrument. The instrument works according to the parameters set by the factory. The digital tube displays the actual speed and temperature. If it needs to be cooled, press the cooling key, and the temperature digital tube displays the actual temperature in the cavity. (4) If you need to adjust the operating parameters of the instrument: 1) Set the running time and running speed: press the function key to make the corresponding indicator light, the digital tube will display the parameter value, and a digit in the digital tube flashes. At this time, "", "" and "" can be used. Adjust the parameter to the desired value by pressing the key and confirm the storage by pressing the memory key. 2) Set the temperature: press the cooling function key to make the corresponding (upper or lower limit) indicator light, the digital tube will display the parameter value, and a digit in the digital tube is flashing. At this time, "", "" and "" can be used. Press the key to adjust the parameter to the desired value, and press the cooling function key again to exit the setting program. (5) During the operation of the instrument, the digital tube displays the speed. When you need to view other parameters, press the function key to make the indicator light of the parameter light, and the digital tube displays the value. When the instrument reaches the set time or stops midway, the digital tube displays the speed during the stop, which is normal. Second, note: 1. To ensure safety and centrifugal effect, the instrument must be placed on a solid horizontal surface, and no items should be placed on the engineering plastic door; the samples must be placed symmetrically and ensure that the nuts are tightened before starting the machine; 2. Always check whether the rotor and the experimental centrifuge tube have cracks and aging, and if necessary, replace them in time; 3. After the experiment is completed, the instrument should be wiped clean to prevent corrosion; 4. If the sample specific gravity exceeds 1.2g/cm3, zui high speed n=nMax×SQRT (1.2/sample specific gravity); 5. When the length of the brush is less than 6mm, it must be replaced in time; 6. Do not open the door when the centrifuge is not stationary; 7. The instrument must have a reliable grounding; 8. If the instrument is used in an unstable voltage region, a voltage regulator should be added to ensure that the instrument performs well. 9. Plug in the power, turn on the power switch, the digital tube display of the instrument control system, please press the stop button, the speed digital tube displays OPEN, the electronic door lock action, the door is automatically lifted slightly, then the door can be opened by hand, such as cover The door cannot be automatically lifted. The door must be opened by hand within 3 seconds. The electronic door lock automatically locks the door after more than 3 seconds. If the door is opened, you must press the stop button speed on the panel to display OPEN to open the door. 10. If the user does not make any changes to the factory-set parameters, please place the test tube with the same amount of test sample into the rotor hole symmetrically and cover the door. Then press the run button to run directly. To change the setting parameters, press the key on the panel to set and remember. After the completion, press the operation key to operate the instrument. At the end of the operation, 0 to OPEN is displayed. At this time, the door can be opened. If the door is not opened within 3 seconds and OPEN becomes 0, press the stop button to display OPEN, and open the door within 3 seconds. Portable 3 Ply Disposable Masks Non-woven Face Mask With Elastic Earloops Disposable Surgical Face Mask Dongguan Anmeixuan Electronic Technology Co., Ltd , https://www.hyashine.com
In addition, the substance is accompanied by a diffusion phenomenon when it settles in the medium. Diffusion is unconditional and absolute. Diffusion is inversely proportional to the mass of the material, and the smaller the particle, the more severe the diffusion. The settlement is relative, and if it is conditional, it must be subjected to external forces to move. The settlement is proportional to the weight of the object, and the larger the particle, the faster the sedimentation. For particles smaller than a few micrometers such as viruses or proteins, they are in a colloidal or semi-colloidal state in solution, and it is impossible to observe the sedimentation process by gravity alone. The smaller the particles, the slower the sedimentation, and the more severe the diffusion phenomenon. Therefore, it is necessary to use a centrifugal machine to generate a strong centrifugal force in order to force these particles to overcome the diffusion to produce a settling motion.
Centrifugation is the use of a powerful centrifugal force generated by the high-speed rotation of the centrifuge rotor to accelerate the sedimentation velocity of the particles in the liquid, separating the sedimentation coefficients and buoyancy-density materials in the sample.
Second, the classification of laboratory centrifuges:
There are three classification methods for centrifuges: by purpose, by speed, and by structure.
â—Classify by use: It is divided into preparative centrifuge and preparation analytical centrifuge.
Preparative centrifuge: A centrifuge that only separates and concentrates and purifies the sample.
Preparation and Analysis Centrifuge: It can not only separate and concentrate the sample, but also can observe, take photos, measure, digital output and print the centrifuge of the sample through the optical system.
â— Classification by speed: divided into low speed centrifuge, high speed centrifuge, ultracentrifuge.
Low speed centrifuge: The speed is within 10000 rpm or the relative centrifugal force is within 15000 × g.
High-speed centrifuge: The rotation speed is within 10000∽30000rpm or the relative centrifugal force is within 15000∽70000×g.
Ultracentrifuge: The rotation speed is above 30,000 rpm or the relative centrifugal force is above 70,000 × g.
â— Classified by structure:
Generally, high-speed and low-speed centrifuges are classified according to structure and function, and there are many varieties, and there is no uniform standard for each manufacturer. Can be divided into desktop centrifuge, multi-tube micro-tabletop centrifuge, cell smear centrifuge, blood washing desktop centrifuge, high-speed refrigerated centrifuge, large-capacity low-speed refrigerated centrifuge, low-speed refrigerated centrifuge, desktop high-speed refrigerated centrifuge, desktop Low-speed automatic balancing centrifuges, etc. In addition, there is a triple-type (five-type) high-speed refrigerated centrifuge in foreign countries, which is specially used for continuous centrifugation.