China is the world's largest producer of rapeseed, with annual output ranging from 600,000 to 7 million tons. This production generates a significant amount of rapeseed meal, which is considered an excellent natural vegetable protein source. Its protein content and amino acid profile are comparable to that of soybean meal. However, rapeseed meal contains toxic or anti-nutritional compounds such as glucosinolates, phytic acid, and tannins, which can lead to poisoning in monogastric animals and reduce feed palatability. To address these issues, several detoxification methods have been developed, including physical, chemical, and biological approaches. The physical detoxification method primarily focuses on inactivating the enzyme myrosinase. However, recent studies show that glucosinolates themselves are not entirely harmless, and some microorganisms in the gut can produce similar enzymes to break them down into toxic byproducts. This limits the effectiveness of physical methods alone. Chemical detoxification involves processes like acid-base degradation, metal salt catalysis, and solvent leaching. Among these, the double-liquid extraction method is the most widely researched. It can achieve a detoxification rate of over 90%. However, it suffers from high costs, dry matter loss, and environmental pollution, making it less practical for large-scale use. Biological detoxification includes enzyme-catalyzed hydrolysis and microbial fermentation. Enzyme-based methods often involve adding specific activators, such as vitamin C, to speed up the breakdown of glucosinolates. Solvents are then used to remove the toxins. While this approach can also eliminate phytic acid and polyphenols, it is limited by the difficulty in obtaining the necessary enzymes and the high cost and complexity of the process, which hinders industrial application. Microbial fermentation, on the other hand, offers a more promising solution. It uses microorganisms to directly break down harmful substances. This method is characterized by mild conditions, a simple process, and minimal dry matter loss. For example, using a microbial starter like Jinbao Feed Starter, you can mix corn flour, wheat bran, rice bran, and rapeseed cake powder in a ratio of 20:30:10:40. Add the starter at 20% of the total weight, mix thoroughly, and add water to reach about 65% moisture. Place the mixture in sealed containers such as jars or plastic bags and ferment for two to three days. Once the fermentation is complete, the treated meal becomes highly palatable to pigs and chickens. It can be gradually introduced into full-price feed, replacing up to 40–50% of the feed without affecting animal health. This significantly reduces feed costs and provides an economical and sustainable feeding option. Shandong YingLang Chemical Co.,Ltd , https://www.sdylhgtrade.com