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The bottom of the test tube, which is to be stored at the bottom of the well, is sealed with a sterile rubber plug and paraffin. It is sealed in a sealed large-mouth bottle or plastic bag. It is submerged in the bottom hole and can be stored for 2 to 5 months.
Paraffin sealing storage Cut the wax paper into 6 to 8 cm squares and soak it in 75% alcohol for 1 minute. After the mouth of the tube species that has been cultured is rotated and sterilized above the flame of the alcohol lamp, the wax paper is taken out with tweezers and the nozzle is tightened. The stenciled paper nozzle is then immersed in molten paraffin, and the wax paper is completely immersed so that the wax paper is tightly packed on the wall of the tube and no air leakage occurs. After the paraffin cools and solidifies, it is stored in the refrigerator or at room temperature.
Cellophane sealed gelatin 15 grams (binder), copper sulfate 2 grams, 1 to 2 drops of methylene blue (indicator), dissolved in 100 ml of hot water, formulated as a sealing agent. Remove the newly grown mother-of-pearl tampon, face down, place a sealing agent, attach cellophane soaked in 95% alcohol, and seal with paraffin wax. At room temperature, strains can be stored for at least 8 months and can prevent the infestation of molds or maggots. Injecting sterile mineral oil (liquid paraffin) on the slope can greatly extend the storage time.
Bacterial strain effective storage method
The preservation of edible fungus strains can effectively maintain its superior species and extend the years of use of fine varieties. Therefore, it is of equal importance to the selection and breeding of new varieties and is an indispensable and important link in the production of edible fungi.