Kit instruction manual This kit is for research use only.                                           48T purpose of usage: This kit qualitatively measures parvovirus ( CPV ) in pig blood, or other related tissues . Experimental principle: This kit uses double antibody sandwich enzyme-linked immunosorbent assay ( ELISA ) to determine porcine parvovirus ( CPV ) in specimens . Purified porcine parvovirus (CPV) antibody-coated microtiter plate wells the immobilized antibody, the sample may be combined with parvovirus (CPV) antigen, and then after washing to remove unbound antibodies and other components, and HRP-labeled The parvovirus ( CPV ) antibody binds to form an antibody - antigen - enzyme - labeled antibody complex, which is thoroughly washed and then added to the substrate TMB for color development. TMB in HRP enzyme blue, yellow and eventually converted to the action of an acid. The absorbance ( OD value) was measured at 450 nm using a microplate reader , and compared with the CUTOFF value to determine the presence or absence of porcine parvovirus ( CPV ) in the specimen . Kit composition: 1 20 times concentrated washing solution 20ml × 1 bottle 7 Stop solution 3ml × 1 bottle 2 Enzyme standard reagent 3ml × 1 / bottle 8 Positive control 0.5ml × 1 bottle 3 Enzyme label coated plate Article 12 holes × 4 9 Negative control 0.5ml × 1 bottle 4 Sample diluent 3ml × 1 bottle 10 Instruction manual 1 copy 5 Developer A solution 3ml × 1 bottle 11 Sealing film 2 sheets  6 Developer B solution 3ml × 1 bottle 12 sealed bag 1 Specimen requirements: 1 . Specimen processing: serum and plasma samples can be directly detected 2 . The specimens were tested as soon as possible after preparation. If it cannot be detected in time, the specimen can be 3. Samples containing NaN3 could not be detected because NaN3 inhibited horseradish peroxidase ( HRP ) activity. Steps: 1.         No: Samples corresponding to micropores numbered sequentially, each plate hole 2 should be set negative control, 2 positive control wells, blank control hole (blank control wells do not add sample and HRP, other each step operation) 2.         Loading: Negative control and positive control (standard) 50 μl were added to the negative and positive control wells, respectively . Then add 40 μl of the sample dilution to the sample well to be tested , and then add 10 μl of the sample to be tested . Add the sample to the bottom of the well of the microplate, try not to touch the wall of the well, and shake it gently. 3.         Incubation: sealing with a sealing film 4.         Dosing: 20 times concentrated washing solution diluted with distilled water 20 times and used 5.         Washing: Carefully remove the sealing film, discard the liquid, dry it, fill each well with the washing solution, let stand for 30 seconds, then discard it, repeat 5 times, and pat dry. 6.         Add enzyme: 50 μl of enzyme labeling reagent was added to each well , except for blank wells. 7.         Incubation: The operation is the same as 3 . 8.         Washing: The operation is the same as 5 . 9.         Color development: Add 50 μl of developer A to each well , then add 50 μl of developer B , gently shake and mix. 10.     Termination: 50 μl of stop solution was added to each well to terminate the reaction (when the blue color turned yellow). 11.     Measurement: The absorbance ( OD value) of each well was measured in sequence with a blank air conditioner of zero and a wavelength of 450 nm . The measurement should be carried out within 15 minutes after the addition of the stop solution . The result is judged:   Test validity: mean value of positive control well ≥ 1.00; mean value of negative control ≤ 0.15  Criteria ( CUT OFF ) calculation: Threshold = negative control well average + 0.15  Negative judgment: sample OD value < critical value ( CUT OFF ) is negative for parvovirus ( CPV )  Positive judgment: sample OD value ≥ critical value ( CUT OFF ) is parvovirus ( CPV ) positive Precautions 1 . The operation is carried out in strict accordance with the instructions. The components of the different batches of this reagent shall not be mixed. 2 . The kit should be taken out from the refrigerated environment and should be used at room temperature for 15-30 minutes before use. If the enzyme label is unsealed after opening, the slats should be stored in a sealed bag. 3 . Concentrated washing liquid may crystallize out. When diluted, it can be heated and dissolved in a water bath. The washing will not affect the result. 4.  The sealing film is intended for single use only to avoid cross-contamination. 5 . Please keep the substrate away from light. 6 . The test results must be determined by the microplate reader. When using dual-wavelength detection, the reference wavelength is 630nm. 7 . All samples, washings and various wastes should be treated as infectious materials. Stop solution Storage conditions and expiration date 1 . The kit of preservation:; 2 2 . Validity: 6 months Fresh Carrots,Bulk Fresh Carrot,Best New Fresh Carrot,Premium Fresh Organic Carrots Yirun Agricultural Cooperative , https://www.yiruncn.com